![]() ![]() Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes. Expression of c-KIT and its ligand, stem cell factor, in normal and subfertile human testicular tissue. Effects of monoclonal anti-c-kit antibody (ACK2) on melanocytes in newborn mice. A model to study the phenotypic changes of interstitial cells of Cajal in gastrointestinal diseases. Cardiomyogenic potential of C-kit +-expressing cells derived from neonatal and adult mouse hearts. c-kit + precursors support postinfarction myogenesis in the neonatal, but not adult, heart. Adult c-kit pos cardiac stem cells are necessary and sufficient for functional cardiac regeneration and repair. ![]() Clonality of mouse and human cardiomyogenesis in vivo. Adult cardiac stem cells are multipotent and support myocardial regeneration. Haematopoietic stem cells do not transdifferentiate into cardiac myocytes in myocardial infarcts. Haematopoietic stem cells adopt mature haematopoietic fates in ischaemic myocardium. Bone marrow cells regenerate infarcted myocardium. Thus, endogenous c-kit + cells can generate cardiomyocytes within the heart, although probably at a functionally insignificant level. ![]() By contrast, c-kit + cells amply generated cardiac endothelial cells. Endogenous c-kit + cells did produce new cardiomyocytes within the heart, although at a percentage of approximately 0.03 or less, and if a preponderance towards cellular fusion is considered, the percentage falls to below approximately 0.008. A complementary DNA encoding either Cre recombinase or a tamoxifen-inducible MerCreMer chimaeric protein was targeted to the Kit locus in mice and then bred with reporter lines to permanently mark cell lineage. Here we generated two genetic approaches in mice to examine whether endogenous c-kit + cells contribute differentiated cardiomyocytes to the heart during development, with ageing or after injury in adulthood. c-kit-expressing cardiac progenitor cells have been reported as the primary source for generation of new myocardium after injury. If and how the heart regenerates after an injury event is highly debated. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |